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. 2020 Mar 4;10(10):4334–4348. doi: 10.7150/thno.42982

Figure 4.

Figure 4

miR-128 regulates osteoclastogenesis by targeting SIRT1 and NF-κB signaling. A Efficiency and specificity of the SIRT1 probe by qRT-PCR. Data are presented as mean ± SD. **P < 0.01 by two-way ANOVA. B miR-128 could bind to SIRT1 mRNA, as shown by pull down assays. The expression of miR-128 was detected by qRT-PCR. Data are presented as mean ± SD. **P < 0.01 by Student's t test. C Schematic illustrations of the hypothetical duplexes formed by miR-128 with the 3' UTR of SIRT1. D Luciferase activities as quantified from the BMMs co-transfected with the WT or mutant 3' UTR of SIRT1 luciferase reporter plasmids together with miR-128 inhibitor or mimic and or the corresponding control. Data are presented as mean ± SD. **P < 0.01 by two-way ANOVA. E Western blot analysis of SIRT1, p65-Acetyl 310, and p65 protein levels in BMMs derived from WT and miR-128Oc-/- mice. F mRNA expressions of Tnf-α and IL-1 in BMMs derived from WT and miR-128Oc-/- mice were analysed via qRT-PCR analysis. Data are presented as mean ± SD. **P < 0.01 by Student's t test.