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. 2020 Mar 4;10(10):4334–4348. doi: 10.7150/thno.42982

Figure 5.

Figure 5

The decrease of osteoclastogenesis by osteoclastic miR-128 deficiency was due to its inhibitory effect on SIRT1. A Osteoclast differentiation as observed in BMMs derived from WT and miR-128Oc-/- mice that were transfected with the control siRNAs (siCtrl) or siRNA-targeted SIRT1 (siSIRT1) and stimulated with RANKL for three days. TRAP staining was performed. Scale bars: 200 μm. B Osteoclast number and size. TRAP-positive cells with at least three nuclei were counted as osteoclasts. Data are presented as mean ± SD. **P < 0.01 by two-way ANOVA. C Western blot analysis of p65-Acetyl 310 and p65 protein levels in BMMs derived from WT and miR-128Oc-/- mice that were transfected with the siCtrl or siSIRT1. D, E qRT-PCR analysis of mRNA expression of Tnf-α and IL-1 in BMMs derived from WT and miR-128Oc-/- mice that were transfected with the siCtrl or siSIRT1. Data are presented as mean ± SD. **P < 0.01 by two-way ANOVA. F qRT-PCR analysis of mRNA expression of Nfatc1, Traf6, Ctsk, c-Fos, and c-Src in BMMs derived from WT and miR-128Oc-/- mice that were transfected with the siCtrl or siSIRT1. Data are presented as mean ± SD. **P < 0.01 by two-way ANOVA.