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. 2020 Mar 4;10(10):4334–4348. doi: 10.7150/thno.42982

Figure 6.

Figure 6

Osteoclastic miR-128 deficiency prevents mice from OVX-induced bone loss. A A schematic diagram depicting the procedural steps. B-G OVX or sham surgery was done on 10-week-old female WT and miR-128 miR-128Oc-/- mice. Mice were then analysed at 5 weeks post-procedure. B Uterine weights of WT and miR-128Oc-/ mice with sham or OVX surgery. Data is depicted in terms of mean ± SEM. **P < 0.01 by two-way ANOVA. C Representative images of micro-CT reconstruction (Scale bars: 500 μm) and D representative TRAP-stained sections (yellow arrows: osteoclasts) of Tr.B of the L1 vertebrae harvested from the WT and miR-128Oc-/- mice with sham or OVX surgery (n = 8/group) (Scale bars: 200 μm). E, F Histomorphometry analysis of the L1 vertebrae metaphyseal region dissected from 10-week-old littermate male WT and miR-128Oc-/- mice with sham or OVX surgery. Data is depicted in terms of mean ± SEM. **P < 0.01 by two-way ANOVA. G Serum TRACP-5b values tested by ELISA from 10-week-old littermate male WT and miR-128Oc-/- mice with sham or OVX surgery. Data is depicted in terms of mean ± SEM. **P < 0.01 by two-way ANOVA. H Model of the novel identified miR-128/SIRT1/NF-κB axis in osteoclastogenesis and osteoporosis.