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. 2020 Mar 15;10(10):4395–4409. doi: 10.7150/thno.43239

Figure 3.

Figure 3

circPRRC2A promotes the migration and invasion of renal cancer cells in vitro. A Schematic illustration showed two targeted shRNAs and circPRRC2A expression vectors B circPRRC2A-shRNA targets the back-splice junction of circPRRC2A. C qRT-PCR for PRRC2A and circPRRC2A mRNA in ACHN cells treatedwith two shRNAs as described above. D qRT-PCR for PRRC2A and circPRRC2A mRNA in 786-o cells transfected with control vector or circPRRC2A over-expression plasmid. E-G Growth curves of RCC cells were measured after transfection with indicated vectors by Cell Counting Kit-8 assays (CCK-8). H-I Wound-healing assays showed that knockdown of circPRRC2A inhibited the migration ACHN and Caki-1 RCC cells. J Transwell assays showed that knockdown of circPRRC2A inhibited the metastasis of ACHN and Caki-1 cells. Wound-healing K and transwell assays L showed that overexpression of circPRRC2A promoted the invasion ability of the 786-o cells. Data indicate mean ± SD, n = 3; *P < 0.05, **P < 0.01.