Figure 7.

RNA-sequencing showed circPRRC2A could promote the angiogenesis and metastasis of RCC through the miR-514a-5p/miR-6776-5p/TRPM3 pathway. A Expression profile of circPRRC2A-regulated oncogenes using the RNA-seq in ACHN/Caki-1/sh-CTL and ACHN/Caki-1/sh-circRNA cells. B-C A volcano map shows differentially expressed genes (DEG). There is a significant overlap of circPRRC2A associated gene expression changes in ACHN and Caki-1 cell lines. D RNA-seq analyses were performed in triplicate on ACHN and Caki-1 cells. The datasets were analyzed by GSEA, using the Hallmark gene signature collection. GSEA indicating significant correlations between circPRRC2A expression and tumor metastasis-related gene signatures. E RNA-seq analyses of circPRRC2A knockdown in RCC cell lines, significantly enriched Hallmark_EMT pathways in both cell types. F The expressions of TRPM3, VEGFA and EMT-related makers and stemness-related proteins were determined using western blot. Proteins were isolated from cells transfected as indicated. Data indicate mean ± SD, n = 3. G Molecular mechanism of circPRRC2A. PRRC2A gene amplification leads to increased expression of circPRRC2A in mRCC tissues. circPRRC2A sponges miR-514a-5p and miR-6776-5p, thereby regulating angiogenesis and EMT processes via increasing TRPM3 activity in RCC cells' invasion and metastasis. *P < 0.05, **P < 0.01. GSEA, gene set enrichment analysis, EMT, Epithelial-Mesenchymal Transition.