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. 2020 Mar 26;10(10):4705–4719. doi: 10.7150/thno.42417

Figure 5.

Figure 5

CircSAMD4A promotes adipogenesis via the miR-138-5p/EZH2 pathway. Quantification of EZH2 expression by qRT-PCR following (A) miR-138-5p knockdown or (B) circSAMD4A overexpression. Quantification of EZH2 expression by qRT-PCR following transfection with (C) circSAMD4A-specific siRNAs or (D) miR-138-5p mimic was quantified with qRT-PCR. Luciferase assay where preadipocytes were co-transfected with (E) scrambled control, circSAMD4A expression plasmid, and a luciferase reporter plasmid containing either wild-type EZH2 (EZH2-WT) or (F) EZH2 construct with mutated miR-138-5p binding sites (EZH2-mut). (G-H) Reversion assays using vectors overexpressing or konckdown circSAMD4A, as well as miR-138-5p mimics or inhibitors. Data are presented as means ± SD; significant difference was identified with Student's t test. *P < 0.05; **P < 0.01; ns (not significant).