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. 2020 Mar 26;10(10):4705–4719. doi: 10.7150/thno.42417

Figure 6.

Figure 6

AAV9-mediated circSAMD4A downregulation counteracts HFD-induced obesity. (A) Administration of AAV9 vectors through tail vain injection of mice. (B) C57BL6 mice were fed HFD for 8 weeks and then administered with AAV-si-mmu_circ0000529 vectors (AAV-si-mmu_circ). Control mice received AAV-Scramble. Representative images of the HE staining of the VAT from mice administered with either AAV vectors. (C) Body weight in C57BL6 mice treated with AAV-si-mmu_circ or AAV-Scramble. (D) Representative images of the mice administered with AAV-si-mmu_circ or AAV-Scramble. (E) Glucose tolerance was determined after the intraperitoneal injection of glucose. (F) Insulin sensitivity was determined after an intraperitoneal injection of insulin. (G) Fat and lean mass of circRNA knockdown and control mice were determined. (H) Food intake, (I) O2 (J) CO2 production and (K) Heat generation consumption were measured during dark and light cycles in a 24-hour feeding period in metabolic chambers. Data are presented as means ± SD; significant difference was identified with Student's t test. *P < 0.05; **P < 0.01. Scale bar = 100µm.