Skip to main content
. 2020 Mar 15;10(10):4453–4465. doi: 10.7150/thno.42354

Figure 1.

Figure 1

In syngeneic orthotopic liver transplantation subjected to extended cold storage (in 4°C UW solution for 20 h), there were three experimental groups (n = 12/group) of recipients treated with PBS, PACAP+DMSO or PACAP+3-MA at liver harvest and immediately prior to reperfusion through portal vein injection. (A) OLT survival rate was monitored during 14 days post-OLT: PBS (41.7%, red); PACAP+DMSO (91.7%, green, p<0.001); and PACAP+3-MA (50%, blue). Recipients were sacrificed at 6 h and 24 h post-transplant and OLT/serum samples were collected. (B) sALT levels; (C) liver pathology (H&E staining; magnification: x100: scale bar represents 200µm; x400: scale bar represents 20µm); (D) western blot of Atg5, LC3 I, LC3 II p62, PCNA, and Ki67; (E) qPCR-assisted detection of LC3a, LC3b, Beclin-1, EGF, HGF, and c-Met; (F) electron microscopy imagine of graft at 6 h post of OLT (red arrow indicated autophagosome, scale bar represents 0.5µm) (*p<0.001, n=4-6/group).