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. 2020 Mar 15;10(10):4453–4465. doi: 10.7150/thno.42354

Figure 3.

Figure 3

PACAP-CREB axis in liver IRI. (A) Western blot of pCREB in cold storage stressed OLT; (B) representative immunofluorescence staining of pCREB (green), F-actin (red) and DAPI (blue): pCREB accumulation in nuclear (magnification: x400, scale bar represents 20µm). PACAP+DMSO, PACAP+CREB inhibitor or PBS pre-treated WT mice were subjected to partial liver warm ischemia (90 min). Ischemic liver tissues were harvested at 6 h of reperfusion for (C) sALT levels; (D) liver pathology (H&E staining; magnification: x100: scale bar represents 200µm; x400: scale bar represents 20µm); (E) qPCR of TNF-α, IL-1β, CXCL-10 and IL-10 (*p<0.001, n=4-6/group).