Skip to main content
. 2020 Mar 15;10(10):4453–4465. doi: 10.7150/thno.42354

Figure 5.

Figure 5

KLF4 in PACAP-CREB axis controls hepatocellular function in liver IRI. (A) qPCR and western blot of KLF4 in ischemic livers (*p<0.001); (B) Representative immunofluorescence staining of KLF4 (green) and F-actin (red): nuclear KLF4 accumulation (magnification: x400, scale bar represents 20µm). PACAP+DMSO, PACAP+KLF4 inhibitor or PBS pre-treated WT mice were subjected to partial liver warm ischemia (90 min). Ischemic liver tissues were harvested at 6 h of reperfusion for (C) sALT levels; (D) liver histology (representative H&E staining; magnification: x100: scale bar represents 200µm; x400: scale bar represents 20μm); (E) qPCR-assisted detection of TNF-α, IL-1β, CXCL-10 and IL-10 (*p<0.001, n=4-6/group).