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. 2020 Mar 15;10(10):4466–4480. doi: 10.7150/thno.42478

Figure 8.

Figure 8

The cytotoxicity effect of SR9009 was mediated by REV-ERBα autophagy inhibition. (A) H69AR and H446DDP cells transfected with si-REV-ERBα or negative control vector were incubated with SR9009 for 48 h. The protein expression of autophagy associate protein LC3-I/II and P62 was measured by western blot analysis. (B) LC3II/I and P62 expression were detected by western blot analysis, after transfected with a plasmid encoding Atg5 or the corresponding negative control vector. (SR9009 treatment in H69AR and H446DDP cells, 10 µm). (C) CCK-8 assays showed the effect of upregulation of Atg5 on cytotoxicity induced by SR9009 (SR9009 treatment in H69AR and H446DDP cells, 10 µm). The data are presented as means ± SD of three independent experiments. **P < 0.01.