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. 2020 Mar 15;10(10):4589–4605. doi: 10.7150/thno.34065

Figure 8.

Figure 8

TLR9-p38 pathway involvement in mtDNA- and CBNP-induced pulmonary inflammation. (A) ODN2088 (25 µg) pretreatment inhibited p38 phosphorylation of neutrophils induced by mtDNA in vitro. (B) Mitochondrial DNA (2.5 µg) with the pretreatment of ODN2088 or ODN Control (50 µg/mouse) was instilled into wide-type C57BL/6 mice. H&E staining of mouse lung sections 24 h after instillation is shown. Scale bar = 20 µm. (C) The representative lung sections for the H&E staining of wild-type C57BL/6 mice and Tlr9-/- mice are shown. Scale bar = 20 µm. (D-E) The influxes of neutrophils in the whole lungs of wild-type C57BL/6 mice and Tlr9-/- mice were detected 24 h after mtDNA instillation. (F-G) The influxes of neutrophils in the whole lungs of wild-type C57BL/6 mice and Tlr9-/- mice were detected 24 h after CBNPs instillation. (H) Prior instillation of CBNPs, mice were pretreated with SB 203580, 24 h later the influxes of neutrophils in the whole lungs were detected. Data are mean ± SEM; n = 3-4 per group. *P < 0.05 by Student's t-test.