Figure 9.

The released mtDNA induces LTB4 production from macrophages. (A-B) C57BL/6 mice were instilled with saline containing 0.2 mg of CBNPs or mtDNA (2.5 µg). After 6 h of treatment, the supernatant of the BALF was collected for LTB4 determination (n = 3 per group). (C) Bone marrow-derived macrophages (BMDMs) were primed with LPS (10 ng/ml) for 3 h and then treated with mtDNA (2.5 µg) for 6 h. The cell culture supernatants were collected for LTB4 determination. Data are mean ± SEM; **P < 0.01, ***P < 0.001 by Student's t-test.