Figure 6.

YAP interacts with RUNX1 to transcriptionally regulate HDAC2 expression in BC cells. (A) HDAC2 mRNA and protein levels in related cell lines with SRGN knockdown or YAP knockdown were examined by qRT-PCR and Western blotting, respectively. Student's t-test, mean ± s.d. (n=3), _**** p < 0.0001. (B) HDAC2 mRNA and protein levels in cell lines with combined YAP knockdown and SRGN overexpression were examined by qRT-PCR and Western blotting, respectively. Student's t-test, mean ± s.d. (n=3), _*** p < 0.001. (C) HDAC2 mRNA and protein levels in cell lines with TEAD1 knockdown were examined by qRT-PCR and Western blotting, respectively. Student's t-test, mean ± s.d. (n=3), _**** p < 0.0001. (D) HDAC2 mRNA and protein levels in cell lines with combined TEAD1 knockdown and SRGN overexpression were examined by qRT-PCR and Western blotting, respectively. Student's t-test, mean ± s.d. (n=3), _*** p < 0.001. (E) Luciferase activity driven by the HDAC2 promoter in cell lines with combined RUNX1 knockdown and SRGN overexpression was determined by a reporter assay. Student's t-test, mean ± s.d. (n=3), _** p < 0.01, _*** p < 0.001. (F) Enrichment of YAP at the HDAC2 promoter in cell lines with RUNX1 knockdown was detected by ChIP-qPCR. Student's t-test, mean ± s.d. (n=3), _** p < 0.01, _*** p < 0.001. (G) Interaction between YAP and RUNX1 was examined using a CoIP assay.