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. 2020 Apr 11;7:13. doi: 10.1186/s40580-020-00223-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Optimization of the Cap-Ab adsorption. The Cap-Ab coating efficiency was compared two coating buffers. To detect the PfLDH in the microfluidic microplate, the Cap-Ab was prepared in a carbonate-bicarbonate buffer (pH 9.6) and b PBS (pH 7.4). The chemiluminescence were measured by BioTek multimode reader (Excitation: 530 nm, Emission: 590 nm, n = 3)