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. 2020 Apr 7;12:2471–2479. doi: 10.2147/CMAR.S232260

Figure 5.

Figure 5

Circ-ZNF609 mobilizes miR-1224-3p/ETV1 axis to promote the growth of LUAD cells. (A) qRT-PCR analysis for EVT1 expression in control or circ-ZNF609-overexpressing A549 and SPC-A1 cells after transfection with miR-1224-3p. (B) Western blot analysis for EVT1 protein expression in control or circ-ZNF609-overexpressing A549 and SPC-A1 cells that were transfected with miR-1224-3p. (C) The cell proliferation of control or circ-ZNF609-overexpressing A549 and SPC-A1 cells after being transfected with control or miR-1224-3p-overexpressing vector for 24, 48, 72, 96 h. (D) Colony formation assay for control or circ-ZNF609-overexpressing A549 and SPC-A1 cells transfected with control or miR-1224-3p-overexpressing vector. (E) Flow cytometry analysis revealed the cell-cycle distribution of control or circ-ZNF609-overexpressing A549 and SPC-A1 cells transfected with control or miR-1224-3p. Values are expressed in mean ± SD. **p<0.01.