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. 2020 Mar 31;2020:7091053. doi: 10.1155/2020/7091053

Figure 5.

Figure 5

The miR-122-5p downregulates the expression of PLD1 to promote apoptosis. (a) Screening of candidate regulatory miRNAs for PLD1 in online databases (miRWalk, miRanda, and miTarget). (b) Expression of miR-122-5p was increased after Cd exposure in NRK-52E and HK-2 cells. (c) Regulatory effect of miR-122-5p on PLD1 3′UTR determined with dual-luciferase reporter assay. (d) miR-122-5p repressed mRNA expression of PLD1 while cotransfected with miR-122-5p and overexpression of PLD1 in NRK-52E cells. (e) miR-122-5p repressed protein expression of PLD1 in NRK-52E cells. (f) miR-122-5p promoted apoptosis in NRK-52E cells. Data are represented as mean ± SD, N = 3. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.