Figure 3.
The level of capillary macrophage is increased in HIV-Tg mice at 24 h after LPS administration. (A,C) Macrophage immunostaining (red color) in the lung (panel A) and kidney (panel C) capillaries. Hematoxylin is used for counterstaining (purple nuclear staining). Original magnification is 400X. (B,D) Quantification of lung (panel B) and kidney (panel D) capillary macrophages. Results are shown as percentage of WT-LPS levels. n = 3. (E,F) qRT-PCR for HIV-1 env in peritoneal and lung Mϕs (E, n = 4) and in peritoneal Mϕs incubated for 72 h at 5% and 21% in vitro (F, n = 3). Mouse 18S rRNA was used for normalization. Mean and SD are shown. *p < 0.05.