Figure 3.

The intra-RSE pseudoknot has a read-through-independent role in TCV gRNA accumulation. (A) The intra-RSE pseudoknot. The nts implicated in the pseudoknot are highlighted in blue letters, with the anticipated base-pairing relationship denoted with dotted lines. Three mutants tested, with m3-1 and m3-2 disrupting the pseudoknot, and m3-3 restoring the pseudoknot by combining the mutations in m3-1 and m3-2, are also depicted. (B) A representative Northern blotting showing accumulation levels of the pseudoknot mutants. (C) Box plot quantification of Northern blotting results from multiple repeats.