Figure 4.

The long-distance kissing-loop interaction does not have a read-through-independent role in TCV gRNA accumulation. (A) Diagrams depicting the kissing-loop interaction between the asymmetric loop in RSE and the end loop of the 3′ terminal stem–loop of TCV gRNA, and the mutants tested in this set of experiments. The specific nt changes in these mutants are identical to those in a previous study (see text for details). (B) A representative Northern blotting showing accumulation levels of the mutants. (C) Box plot quantification of Northern blotting results.