Table 3.
Error rates of HIV-1 RT measured in cell-based fidelity assays.
| Assay | RT Mutant | Vector | Reporter Gene/Template | Error Rate (× 10−4 errors/bp/cycle) |
Reference |
|---|---|---|---|---|---|
| Forward mutation | WT (NL4-3) | HIV-1 vector (HIV shuttle 3.12 & 5.1) | lacZα, DNA | 0.34 | [67] |
| Forward mutation | WT (NL4-3) | HIV-1 vector (HIV shuttle 3.12) | lacZα, DNA | 0.40 | [68] |
| Forward mutation | WT (NL4-3) | HIV-1 vector (HIV shuttle 3.12 vpr+) | lacZα, DNA | 0.30 | [69] |
| HIV-1 vector (HIV shuttle 3.12 vpr ATG−) | 1.20 | ||||
| Forward mutation (SSCP) | WT (HXB2) | HIV-1 vector (pHIV-gpt) | HIV-1 LTR, DNA | 0.92 | [25] |
| WT (NL4-3) | HIV-1 vector (NL4-3gpt) | 0.79 | |||
| Forward mutation | WT (NL4-3) | HIV-1 vector (pNL4-3deltaΔ +cass) | tk, DNA | 0.22 | [70] |
| Forward mutation | WT (NL4-3) | HIV-1 vector (pNL4-3 HIG) | U373-MAGI-X4 cells, DNA | 6.90 | [71] |
| Forward mutation | WT (NL4-3) | HIV-1 vector (pSICO-LZF) | lacZα, DNA | 0.22 | [72] |
| HIV-1 vector (pSICO-LZR) | 0.17 | ||||
| Forward mutation | WT (NL4-3) | HIV-1 vector (pSICO-LZF/R) | lacZα, DNA | 0.14 | [73] |
| Y115F (NL4-3) | 0.37 | ||||
| Q151M (NL4-3) | 0.17 | ||||
| M184I (NL4-3) | 0.21 | ||||
| M184V (NL4-3) | 0.18 | ||||
| Forward mutation | WT (HX2B2) | HIV-1 vector (pSDY-dCK) | HIV env, RNA | 0.36 | [74] |
| HIV Int-vif-vpr, RNA | 0.75 |