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. 2020 Mar 6;12(3):164. doi: 10.3390/toxins12030164

Figure 3.

Figure 3

Loxosceles laeta Class I and II recombinant PLDs (A) Multiple sequence alignment of L. laeta class I phospholipases-D Smase I /LlSicTox-alphaIII1i (GenBank accession number AY093599), Ll1/LlSicTox-alphaIII1ii (DQ369999), LlPLD1 (GU121905), LlPLD2 (GU121906), Ll2/LlSicTox-alphaIII2 (DQ370000) and class II Smase II/LlSicTox-betaIA1 (AY093601). Sequences were aligned using the CLUSTAL X2 program [19]. Amino acid identities are shaded in black. Conservative substitutions are in gray, arrows point to amino acid residues involved in catalysis. The asterisks show cysteine residues. (B) Percent identity matrix showing the similarities among aligned PLDs. Values given by CLUSTAL X2 program [19].