Table 3.
Isolates of Fusarium oxysporum f. sp. dianthi infected with mitovirus FodMV1.
| Host Isolatea | RdRp (% nt Identity)b |
Geographic Originc | Source | Year | Race group Assignation by PCR Patternd |
|---|---|---|---|---|---|
| Fod 124 | 97% | Chipiona(Ca) | Plant | 2009 | R2I |
| Fod 233 | 96% | Chipiona(Ca) | Plant | 2011 | R2I |
| Fod 235 | 95% | Chipiona(Ca) | Plant | 2011 | R2I |
| Fod 237 | 95% | Chipiona(Ca) | Plant | 2011 | R2I |
| Fod 253 | 95% | Chipiona(Ca) | Plant | 2011 | R2I |
| Fod 219 | 95% | Lebrija(Se) | Plant | 2011 | R2I |
| Fod 215 | 97% | Lebrija(Se) | Plant | 2011 | R2I |
| Fod 221 | 95% | Lebrija(Se) | Plant | 2011 | R2I |
| Fod 327 | 96% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 328 | 95% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 330 | 96% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 331 | 97% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 332 | 95% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 333 | 96% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 334 | 97% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 341.1 | 95% | Chipiona(Ca) | Plant | 2012 | R2I |
| Fod 337 | 97% | Chipiona(Ca) | Soil | 2012 | R2I |
| Fod 338 | 95% | Chipiona(Ca) | Soil | 2012 | R2I |
| Fod 340 | 96% | Chipiona(Ca) | Soil | 2012 | R2I |
| Fod 310 | 100% | Colombia | Plant | 2012 | R2I |
| Fod 311 | 100% | Colombia | Plant | 2012 | R2I |
| Fod 313 | 100% | Colombia | Soil | 2012 | R2I |
(a) The dsRNA from each isolate was analyzed by RT-PCR using specific primers FodMV1F/FodMV1R directed to the RdRp sequence of FodMV1. (b) Percentage of identity between the nucleotide sequences of the 0.7 kb amplicon obtained with each isolate and the correspondent sequence of FodMV1. (c) Ca = Cádiz province, Se = Sevilla province. (d) Race-group assignation by molecular markers, as described in Gómez-Lama Cabanás et al. [38]. R2I = race 2 molecular group I.