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. 2020 Feb 28;12(3):270. doi: 10.3390/v12030270

Figure 3.

Figure 3

Examples of recombinant vaccine platforms used for introduction of different antigens in plant viral structures. (A) «launch» vector [51] based on TMV for production of antigen-containing viruses in plants. LB and RB denotes agrobacterial shuttle vector T-DNA region left and right borders; P35S—CaMV promoter; sgP—subgenomic promoter; T—transcription terminator; Rep, MP—TMV replicase and movement protein; pink and turkish arrows denote the relative position of antigen (A) and AlMV coat protein gene (CP), respectively [41]. (B) BaMV-based vector for production of antigen (A)-containing mosaic viruses in plants. sgP—subgenomic promoter; A—antigen-coding sequence; 2A—self-processing peptide sequence; Rep, TGB, CP–replicase, triple gene block and coat protein genes [40]. (C) Bacterial system for antigen introduction in plant VLP structure [28]. Antigen (A) and CMV VLPs are obtained from separate bacterial cultures, purified, derivatized and joined in chemical coupling reaction. CMV CP gene contains T-cell epitope coding sequence (red box).