Table 1.

Method validation parameters for the quantification of DONs in wheat and maize by the proposed UHPLC–MS/MS method.

Type	Compound	Natural Contamination	Spike Level	Accuracy Recovery	Precision (RSD)
		(n = 6, µg/kg)	(µg/kg)	(%)	Interday (n = 6, %)	Intraday (n = 3, %)
	DON	14.13	50.00	100.96	10.37	9.23
			100.00	81.08	5.73	4.92
			200.00	82.34	6.61	10.44
wheat	3-ADON	ND1	50.00	104.44	3.76	3.98
			100.00	101.31	6.25	7.81
			200.00	82.50	2.95	7.97
	15-ADON	ND	50.00	103.15	4.10	10.47
			100.00	83.83	12.27	10.01
			200.00	71.72	5.36	6.07
	DON	16.45	50.00	93.50	10.52	7.94
			100.00	90.76	5.53	7.68
			200.00	92.25	5.71	7.89
maize	3-ADON	ND	50.00	103.93	7.80	6.87
			100.00	101.65	13.98	10.3
			200.00	91.72	2.83	4.12
	15-ADON	ND	50.00	112.81	2.26	8.62
			100.00	79.26	4.63	4.63
			200.00	74.55	8.62	6.43

¹ ND-Not detected (<LOD).