Figure 1.

Effect of bee venom phospholipase A2 (bvPLA2) treatment on CD4⁺CD25⁺Foxp3⁺ Treg populations in in vitro splenocyte cultures. Single cell suspension of splenocytes were isolated from Foxp3EGFPC57BL/6 mice and stimulated with PBS, bvPLA2, and rapamycin in the presence of anti-CD3/CD28 antibodies. (A) The gating strategy was depicted. (B) After 72 h, cells were labeled with anti-CD4-PE-Cy7 and anti-CD25-APC-Cy7, and analyzed by flow cytometry. Lymphocytes were initially gated by forward and side scatter properties. CD4⁺ were then gated and assessed for surface expression of CD25 and FoxP3. (C) The percentages of CD4⁺CD25⁺Foxp3⁺ Tregs were depicted as the bar graph. The data are shown as the means ± SEM. Rapa, rapamycin. The significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test. *p < 0.05 vs. the PBS group.