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Effect of bvPLA2 on early apoptosis in splenocytes. Single-cell suspension splenocytes were treated with bvPLA2 or rapamycin for 72 h, stained with Annexin-V, 7-AAD, and active-caspase-3 antibody, and analyzed using flow cytometry. Live cells were gated as 7-AAD negative populations. The early apoptotic cells were depicted as Annexin V and active-caspase-3 double-positive populations.
