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. 2020 Mar 22;12(3):198. doi: 10.3390/toxins12030198

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Involvement of the IL-2 signal in the reduction of apoptosis by bvPLA2 treatment in vitro. CD4⁺ T cells were isolated from mouse splenocytes by negative magnetic-activated cell sorting (MACS) sorting, then labeled with anti-CD25 magnetic beads and isolated. Isolated CD4⁺CD25⁺ cells were treated with bvPLA2 for 72 h in the presence of anti-CD3/CD28 stimulation, then stained with Annexin V, 7-AAD, and active-caspase-3 antibody. Live cells were gated at 7-AAD negative populations. The early apoptotic cells were depicted as Annexin V and active-caspase-3 double-positive populations.