Table 1.

AS-qPCR amplification efficiencies of serial dilutions of DNA samples with distinct proportions of JAK2 mutant alleles.

Mutant Allele % (Dilution)	wt eff 1 (%)	Mut Eff (%)	p Value
96% (1:10)	109.7	106.8	0.61
81.4% (1:2)	104.1	106.9	0.45
62.5% (1:2)	102.4	102.4	0.95
50% (1:10)	109.5	110.02=	0.71
36.9% (1:2)	104.9	104.3	0.91
13.1% (1:2)	102.5	97.9	0.51
6.4% (1:2)	100.5	111.7	0.22

¹ Eff—Amplification efficiencies, slopes of all linear regressions were not different (p = 0.18).