Table 1.
Results of the literature review of studies in which molecular tests were used to diagnose disseminated histoplasmosis.
| Author and Year | n | Samples | Molecular Technique | DNA Extraction | Molecular Target | Probe | Sensitivity | Specificity |
|---|---|---|---|---|---|---|---|---|
| Bialek R et al. (2002) [16] | 100 | Biopsy | Nested PCR | QIAamp tissue kit Qiagen | 18S rDNA Hcp 100 |
None | NA | NA |
| Bracca, A. et al. (2003) [17] | 30 | Blood, biopsy, scraping from mucocutaneous lesions | Semi-nested PCR | Manual extraction | β-glucosidase | None | NA | NA |
| Guedes, H.L. et al. (2003) [18] | 31 | Fungal cultures | DNA Sequencing | Puregene DNA Kit Gentra.Systems | M Protein | None | 100% | 100% |
| Muñoz, C. et al. (2009) [19] | 146 | Fungal cultures | Nested PCR | DNA Minikit Qiagen | Hcp 100 | None | 100% | 92% |
| Muñoz, B. et al. (2010) [20] | 6 | Fungal cultures | Conventional PCR | Manual extraction | M Protein | None | NA | NA |
| Frías-De-León, M. G. et al. (2012) [21] | 40 | Fungal cultures | (RAPD)-PCR | NA | SCAR Markers | None | NA | 100% |
| Ohno, H. et al. (2013) [22] | 7 | Fungal cultures and cinical samples * | Nested PCR | NA | M Protein | None | NA | NA |
| Dantas, K. C. et al. (2013) [23] | 40 | Whole blood and serum | Nested PCR | NA | 18S rDNA Hcp 100 |
None | 96%–98% | HC100 (97%-Whole Blood, 95%-Serum) e HC18S (90% Whole Blood, 88% Serum) |
| Buitrago, M. J. et al. (2013) [24] | 10 | Fungal cultures | Nested, Real Time and Conventional PCR | DNeasy Plant MiniKit Qiagen | Hcp 100, SCAR, ITS1 | Taqman HcITS-155 |
SCAR–43% Hcp 100 - 89.5 |
100% |
| Scheel, C. M. et al. (2014) [12] | 50 | Fungal cultures | Nucleic acid amplification via LAMP | Qiagen Dneasy Kit | Hcp100 | None | NA | 100% |
| Gago, S. et al. (2014) [25] | 43 | Fungal cultures and bronchoalveolar lavage | Multiplex real-time PCR | DNeasy Plant MiniKit Qiagen | ITS | None | 90% | 100% |
| Muraosa, Y. et al. (2015) [13] | 19 | Fungal cultures and clinical samples * | Cycling probe-based real-time PCR and Nested real-time PCR | PrepManTM Ultra Life Technologies | NAALADase Gene | Hist1probe3 | Real Time PCR–77% Nested PCR–33% |
NA |
| Brilhante, R. S. et al. (2016) [26] | 16 | Blood | Conventional PCR | CTAB | RYP1 | None | 100% | 100% |
| Frías-De-León, M. G. et al. (2017) [11] | 4 | Serum | Nested PCR | Qiagen Dneasy Kit | Hcp100 | None | NA | NA |
| Dantas, K.C. et al. (2018) [27] | 40 | Blood | Nested PCR | QIAamp Blood DNA Mini Kit | 18S rDNA Hcp 100 |
None | 18%–60% | 85%–100% |
Legend: NA, data not available; RAPD, random amplified polymorphic DNA; LAMP, Loop mediated isothermal amplification; PCR, Polymerase chain reaction; CTAB, cetyltrimethylammonium bromide. * Clinical Samples include blood, serum, biopsy, bronchoalveolar lavage, and scraping.