Figure 2.
Composite 3D image of HH9 and HH14 transgenic quail heart, Tg[PGK1p.H2B-chFP; hUbCp.memb-eGFP] [67,68]. Membrane-GFP label (memb-eGFP) is shown as yellow-green, cell nuclei-cherryFP label (H2B-chFP) is shown as magenta. (A,C) Full heart at HH9 and HH14, respectively; (B,D) Single z-section within the HH9 and HH14 hearts. At HH14, red blood cells (RBCs) can be visualized from the z-section. Scale bars = 100 μm. SV, sinus venosus; VV, vitelline vein; AIP, anterior intestinal portal; RBCs, red blood cells; EnC: Endocardial cells (lining the inner tube); MCs: myocardial cells.