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Construction of ScFvEGFR (single-chain variable fragment of the epidermal growth factor receptor)-IO (iron-oxide) nanoparticles. Uniform 10 nm IO nanoparticles were coated with amphiphilic copolymers modified with short PEG chains. ScFvEGFR proteins were conjugated to the IO nanoparticles mediated by ethyl-3-dimethyl amino propyl carbodiimide (EDAC). Reprinted with the permission from Wiley [97].
