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. 2020 Apr 6;15(2):138–148. doi: 10.18502/jovr.v15i2.6730

Table 4.

Supporting Material - PCR Primers and annealing conditions used in this work


Primer Forward (5'-3') Reverse (5'-3') Tf (bp) Tm (°C)
LOX – P1a GAGACTGAGATACCCGTGCT AGCGGTGACTCCAGATGA 474 58
LOX – P1b TCACAGTACCAGCCTCAGC ATAGCTGGGGACCAGGTG 500 60
LOX– P2 TTTTCACATTGCTTTGCAGT GCTCTTGTCCCACTTCCTAA 398 58
LOX – P3 TAGTTGGGAAAGGAGGATTG GCAATTTTCTCCCTTCAGGT 355 58
LOX – P4 GACTTATGTCCTGGGGAAAA GATAAAAATGTGTGTGCTCTTCA 441 58
LOX – P5 GGAGGTGCTATAAGGCTGAG TTGCTTCCAATACCATGATT 370 58
LOX – P6 TTCAGGGGAAAATATGCAGT TGCTTACAAGAAAGCTGCTG 394 58
LOX - P7 CTTAGGTGGAGGGAAACTGT AAGTCATTTTGGCTCATTCA 485 58
SOD1 - P1 CTCCACATTTCGGGGTTCT ACCCGCTCCTAGCAAAGGT 450 58
SOD1 - P2 CCATCTCCCTTTTGAGGACA CGACAGAGCAAGACCCTTTC 426 58
SOD1 - P3 TGATGCAGGTCAGCACTTTC AAAAGCATTCCAGCATTTGG 344 58
SOD1 - P4 CCATCTTTCTTCCCAGAGCA GAAACCGCGACTAACAATCAA 386 58
SOD1 - P5 TTTGGGTATTGTTGGGAGGA TCTGTTCCACTGAAGCTGTTT 675 59
SOD1 del CAGAAACTCTCTCCAACTTTGC GAGGGGTTTTAACGTTTAGGG 218 59
PCR, polymerase chain reaction