Table 4.
| ||||
Primer | Forward (5'-3') | Reverse (5'-3') | Tf (bp) | Tm (°C) |
LOX – P1a | GAGACTGAGATACCCGTGCT | AGCGGTGACTCCAGATGA | 474 | 58 |
LOX – P1b | TCACAGTACCAGCCTCAGC | ATAGCTGGGGACCAGGTG | 500 | 60 |
LOX– P2 | TTTTCACATTGCTTTGCAGT | GCTCTTGTCCCACTTCCTAA | 398 | 58 |
LOX – P3 | TAGTTGGGAAAGGAGGATTG | GCAATTTTCTCCCTTCAGGT | 355 | 58 |
LOX – P4 | GACTTATGTCCTGGGGAAAA | GATAAAAATGTGTGTGCTCTTCA | 441 | 58 |
LOX – P5 | GGAGGTGCTATAAGGCTGAG | TTGCTTCCAATACCATGATT | 370 | 58 |
LOX – P6 | TTCAGGGGAAAATATGCAGT | TGCTTACAAGAAAGCTGCTG | 394 | 58 |
LOX - P7 | CTTAGGTGGAGGGAAACTGT | AAGTCATTTTGGCTCATTCA | 485 | 58 |
SOD1 - P1 | CTCCACATTTCGGGGTTCT | ACCCGCTCCTAGCAAAGGT | 450 | 58 |
SOD1 - P2 | CCATCTCCCTTTTGAGGACA | CGACAGAGCAAGACCCTTTC | 426 | 58 |
SOD1 - P3 | TGATGCAGGTCAGCACTTTC | AAAAGCATTCCAGCATTTGG | 344 | 58 |
SOD1 - P4 | CCATCTTTCTTCCCAGAGCA | GAAACCGCGACTAACAATCAA | 386 | 58 |
SOD1 - P5 | TTTGGGTATTGTTGGGAGGA | TCTGTTCCACTGAAGCTGTTT | 675 | 59 |
SOD1 del | CAGAAACTCTCTCCAACTTTGC | GAGGGGTTTTAACGTTTAGGG | 218 | 59 |
PCR, polymerase chain reaction |