Figure 5.

Short-term time course of RNA accumulation immediately after treatment with SA. (A) Timeline of experimental procedure with (SA^plus) or without (SA^minus) 20 µM salicylic acid. Two-week-old seedlings were dipped once into the water or 20 µM SA and incubated on fresh media (SA^minus or SA^plus). Up arrows indicate samplings. Accumulations of AtR8 lncRNA as well as WRKY53 and WRKY70 mRNA in Arabidopsis plants were measured by RT-qPCR with ACT2 and ACT8 genes as standards. (B–E) Controls that were treated with just water (SA^minus). (F–I) Seedlings treated with 20 µM SA (SA^plus). Bar graphs represent mean values of three independent assays (≥ 50 plants), and error bars represent ±SE. W53: WRKY53, W70: WRKY70. Significant differences in RNA accumulations levels between the same strain SA^minus and SA^plus (Wt: B and F, atr8: C and G, wrky53: D and H, and wrky70: E and I) are indicated as * p < 0.05, ** p < 0.01 and **** p < 0.0001 (Welch´s t-test).