Figure 7.

Time course of the RNA accumulations soon after germination. (A) Timeline of the experimental procedure. Up arrows indicate samplings. The Wt, atr8, and wrky70 strains were germinated on 0.24% gellan gum media (without SA and P. syringae), and the total RNAs were extracted after the indicated periods (DAG: days after germination) from each strain, and subjected to RT-qPCR with ACT2 and ACT8 genes as standards. (B) Line graphs represent the mean values of three independent assays (≥ 60 plants), and error bars represent ±SE. Significant differences in RNA accumulation levels between Wt and each of the mutants (Wt and atr8, Wt and wrky70) are indicated as * p < 0.05 and ** p < 0.01 (Welch’s t-test).