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. 2020 Feb 13;8(1):12. doi: 10.3390/medsci8010012

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of autophagic cell death by the Muse™ LC3-II detection assay. (a) Histogram of LC3-II intensity in the control and experimental CaSki cultures. (b) Statistical analyses of the LC3-II induction assays in the control and experimental CaSki cultures. Cholecalciferol treatments did not cause significant changes in LC3-II expression between the control and experimental cultures. Experiments were performed in triplicate with three biological repeats. Values are expressed as mean ± SEM (p < 0.05 was considered statistically significant).