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. 2020 Feb 13;8(1):12. doi: 10.3390/medsci8010012

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transmission electron micrographs of the CaSki, control, and low-dose cholecalciferol-treated cultures. Both the medium control (A) and solvent control (B) showed healthy cells with intact cell and nuclear membranes and clearly defined nuclei (N). Mitochondria (encircled) did not show abnormal morphology. Low-dose cholecalciferol treatments of CaSki cultures of 10 ng/mL (C) and 40 ng/mL (D) did not show morphological features consistent with cell death. Scale bar: (A,C) = 5 µm and (B,D) = 2 µm.