Figure 9.

Transmission electron micrographs of CaSki cell cultures treated with 100 and 1000 ng/mL cholecalciferol. The micrographs (A–C) represent cultures treated with 100 ng/mL cholecalciferol, displaying mitochondria with dilated cristae (black arrows), apoptotic bodies (encircled in black), cell membrane blebbing (black arrowhead), and isolated vacuoles (v). Micrographs (D–F) represent cultures treated with 1000 ng/mL cholecalciferol, which show features of nuclear apoptosis, including nuclear fragmentation (N_f) and various stages of karyolysis (encircled white dashed line), membrane budding (black arrowheads), isolated vacuoles (v), and the presence of apoptotic bodies (encircled in black). (F) Terminal apoptotic events, demonstrating a shrunken cell with an amorphous cytoplasm surrounded by multiple apoptotic bodies (encircled in black). Scale bars: (A,B,D,F) = 2 µm and (C) = 1 µm.