Skip to main content
. 2020 Feb 20;6(1):7. doi: 10.3390/ncrna6010007

Figure 1.

Figure 1

nc886 derived fragments are produced in prostate cell lines and exhibit microRNA features. (A,B) Mapping of prostate cell small RNA reads along the sequence of nc886. Reads obtained from small-RNA transcriptomes of non-transformed prostate PrEc and PrSC (GEO id: GSE26970) (A) and tumor DU145, PC-3 and LNCaP cell lines (SRA id: SRP109305 and GEO id: GSE66035) (B) were mapped to nc886. A diagram of nc886 (Refseq (NR_030583.2)), plus additional 10 nt at both ends, is depicted below the plots. Red lines in the plots represent the previously annotated hsa-miR-886-3p and -5p. (C) Predicted secondary structure of nc886 (Refseq (NR_030583.2)) based on maximum free energy (MFE) generated with the RNAstructure software [44]. (D) Association of small RNAs to Argonaute in transcriptomic analysis of DU145 cell line. The graph shows the normalized expression, reads per million (RPM), of small non-coding RNAs in total cellular RNA (TOTAL DU145) and in the Argonaute PAR-CLIP fraction (AGO DU145). Data set available at SRA id: SRP075075. (E) Transcriptomic analysis of small non-coding RNAs of the HCT116 cell line with knockouts for the microRNAs biogenesis proteins DROSHA, EXPORTIN 5 and DICER. Data set available at GEO id: GSE77989. Average value and standard error are shown.