Table 3.

Column chromatography procedure of MH and ME extracts and yields of obtained fractions.

No.	Fractions	Solvent	Code	Amount (g)	Yield (%)
MH extract
1	1–4	H 100%	D1	2.28	14.84
2	5–13	H 100%	D2	1.13	7.36
3	14–20	HE 10%	D3	3.57	23.24
4	21–47	HE 20%	D4	2.63	17.12
5	48–58	HE 30%	D5	0.52	3.39
6	59–69	HE 40%	D6	0.30	1.95
7	70–81	HE 50%	D7	0.59	3.84
8	82–100	HE 60%	D8	0.81	5.27
9	101–130	HE 70% & HE 80%	D9	0.66	4.30
10	131–152	HE 90% & E 100%	D10	1.34	8.72
11	153–163	EM 50% & M 100%	D11	0.43	2.8
ME extract
12	1–11	H 100% & HE 5%	T1	0.51	5.67
13	12–17	HE 5%	T2	0.14	1.56
14	18–21	HE 5%	T3	0.08	0.89
15	22–26	HE 10%	T4	0.14	1.56
16	27–46	HE 10% & HE 20%	T5	0.74	8.22
17	47–50	HE 20%	T6	0.38	4.22
18	51–63	HE 20%	T7	1.05	11.67
19	64–77	HE 30%	T8	0.99	11.00
20	78–90	HE 30% & HE 40%	T9	0.70	7.78
21	91–101	HE 40%	T10	0.47	5.22
22	102–118	HE 50%	T11	0.60	6.67
23	119–137	HE 60% & HE 70%	T12	0.56	6.22
24	138–156	HE 70%	T13	0.37	4.11
25	157–167	HE 80%	T14	0.20	2.22
26	168–175	HE 90%	T15	0.14	1.56
27	176–181	E 100%	T16	0.15	1.67
28	182–185	MeOH 100%	T17	1.13	12.56

H, n-hexane; HE, n-hexane: ethyl acetate (v/v); EM, ethyl acetate: methanol (v/v); E, ethyl acetate; M, methanol.