Figure 5.

Effects of fractions from methanolic extract of Buchenavia tetraphylla leaves (BTFC and BTFD) on the survival of Tenebrio molitor larvae challenged with Escherichia coli 042. (A) Effects of BTFC on the survival of Tenebrio molitor larvae challenged with E. coli 042. (B) Effects of BTFD on the survival of Tenebrio molitor larvae challenged with E. coli 042. The larvae (n = 10/group) received a lethal dose of EAEC 042 and after 2 h were treated with fraction BTFC and BTFD (at 10 mg/kg or 20 mg/kg). Larvae treated with phosphate-saline buffer (PBS) or EAEC 042 were used as negative or positive controls, respectively. In this set of assays, larvae survival was recorded each 24 h. The experiment was repeated three times.