Skip to main content
. 2020 Apr 9;8:e8921. doi: 10.7717/peerj.8921

Figure 1. Androgen induces Twist1 expression in prostate cancer cells and AR is required for this induction.

Figure 1

(A) LNCaP, C81, CWR-22Rv1 and PC3 cells or (B–D) LNCaP cells were grown in 2% DCC-serum with ethanol (−) or 10 nM R1881 (+) for 48 h with (B) transfection of control or AR siRNA, (C) treatment with or without 50 nM Casodex, or (D) transfection with pGL3, Twist1-Luc, or ARE-Luc. (A–C) Twist1 mRNA was measured by qRT-PCR and Twist1 and AR proteins were measured by Western blotting. Note that β-actin was used as a loading control for the Western blots. (D) Luciferase reporter activity was measured standardized to pCH110 (control transfection). The Luciferase activity shown is relative to the activity of pGL3 (−), which was set as 1. Bar graphs represent averages of three independent experiments plus standard deviation. The Student’s T-test was performed to show statistical significance (*p < 0.05, ***p < 0.01), as indicated by the asterisks.