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. 2020 Apr 9;8:e8921. doi: 10.7717/peerj.8921

Figure 2. ETV1 is required for androgen-induced expression of Twist1.

Figure 2

LNCaP cells grown in full serum (A) or treated with ethanol (−) or R1881 (+) (B and C) were infected with Lentivirus expressing scramble or ETV1 shRNA. In (C), ETV1 rescue was performed by transfecting Empty or ETV1 expression plasmid using Lipofectamine® 3000. Expression of Twist1 and ETV1 were measured using qRT-PCR. Similarly, LNCaP cells grown in full serum (D) or treated with ethanol (−) and R1881 (+) (E) were infected with lentivirus expressing Scramble, AR and ETV1 shRNA and expression of Twist1, ETV1, AR and β-actin were measured using Western blots. (F) LNCaP cells grown in 2% DCC for 48 h were treated with ethanol (−) or R1881 (+) for 4, 8 or 24 h and expression of Twist1, ETV1 and PSA was measured using qRT-PCR. Bar graphs represent averages of three independent experiments plus standard deviations. The Student’s T-test was performed to show statistical significance (*p < 0.05, ***p < 0.01), as indicated by the asterisks.