Figure 4. Twist1 is required for androgen-induced migration of prostate cancer cells.

LNCaP cells were transfected with control (Ctrl), AR, or Twist1 siRNA and measured by qRT-PCR for mRNA expression of E-Cadherin (A), N-Cadherin (B), Twist1 (C), or AR (D). The cells were also monitored for cell migration (E and F), which was standardized to no R1881 and control siRNA, which was set as 1. The cells in (F) were measured by Western blotting for expression of E-Cadherin, N-Cadherin, Twist1 and AR (G), which was quantified using ImageJ and shown as a bar graph (H). In (F), cells were grown in 2% DCC-serum with ethanol (−) or 10 nM R1881 (+). Bar graphs represent averages of three independent experiments plus standard deviations. The Student’s T-test was performed to show statistical significance (*p < 0.05, ***p < 0.01), as indicated by the asterisks.