FIGURE 6-25.

Neoplastic effusion. Pleural. T-cell lymphoma. Dog. Same case A-B. A, Cytocentrifugated specimen demonstrating a variably sized round cell population having scant to moderate amounts of basophilic cytoplasm, fine to moderately coarse chromatin with occasional prominent nucleoli, and variable N:C. (Modified Wright; HP oil.) B, Sediment smear displays positive cell surface staining in all lymphoid cells for the CD3 antigen, which supports a clonal population of T-lymphocytes. Note the small, likely normal, lymphocyte below center. Red cells are barely visible beneath the size bar. Rare small normal lymphocyte reacted with CD79a antibody (not shown). (CD3 antibody; HP oil.)

(A and B, Courtesy of Rose Raskin, Purdue University.)