Table 2.
Cryo-EM data collection, refinement and validation statistics
| #1 Spiral conformation (EMD-8794, PDB 5WC0) | #2 Ring Conformation (EMD-8796, 5WCB) | |
|---|---|---|
| Data collection | ||
| Microscope | FEI Titan Krios | FEI Titan Krios |
| Camera | Gatan K2 Summit | Gatan K2 Summit |
| Magnification | 22,500 | 22,500 |
| Voltage (kV) | 300 | 300 |
| Electron dose (e−/Ǻ2) | 51 | 51 |
| Defocus range (μm) | −1.0 to −3.5 | −1.0 to −3.5 |
| Pixel size (Ǻ) | 1.31 | 1.31 |
| Reconstruction and Model Refinement | ||
| Particles | 38,072 | 16,185 |
| Map sharpening B factor (Ǻ2) | −98.36 | −95.45 |
| Resolution (Å) | 4.4 | 6.0 |
| FSCaverage | 0.884 | 0.90 |
| Model composition | ||
| Non-hydrogen atoms | 11477 | 11422 |
| Protein residues | 1660 | 1662 |
| Ligands (ATP) | 6 | 5 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.003 | 0.004 |
| Bond angles (°) | 0.74 | 0.83 |
| Validation | ||
| MolProbity score | 1.77 | 2.18 |
| Clashscore | 3.91 | 6.76 |
| Poor rotamers (%) | 0.00 | 0.00 |
| Ramachandran plot | ||
| Favored (%) | 92.57 | 87.86 |
| Allowed (%) | 7.37 | 12.14 |
| Disallowed (%) | 0.06 | 0.00 |