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. 2020 Apr 7;26(13):1450–1462. doi: 10.3748/wjg.v26.i13.1450

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©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Calpain-2 silencing mitigates dithiothreitol-upregulated calpain-2 expression, caspase-12 activation and apoptosis of BRL-3A cells. BRL-3A cells were transfected with, or without, control or calpain-2 specific siRNA for 48 h and treated with, or without, dithiothreitol (DTT). The relative levels of calpain-2 expression and caspase-12 activation were quantified by Western blot. The percentages of apoptotic cells were quantified by flow cytometry. Data are representative images or expressed as the mean ± SD of each group of cells from three separate experiments. A: Calpain-2 silencing; B: Calpain-2 silencing mitigates DTT-upregulated calpain-2 expression; C: Calpain-2 silencing decreases DTT-induced caspase-12 activation; D, E: Calpain-2 silencing mitigates DTT-triggered apoptosis of BRL-3A cells. ^aP < 0.05, ^bP < 0.01.