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. 2020 Apr 8;13:3025–3037. doi: 10.2147/OTT.S237559

Figure 2.

Figure 2

Effect of PCAT6 on the radiosensitivity of TNBC cells. (A) The expression of PCAT6 was determined in TNBC cells (MDA-MB-468 and MDA-MB-231) and breast epithelial cells (MCF-10A) using qRT-PCR. (B) The knockdown efficiency of PCAT6 was examined in MDA-MB-468 and MDA-MB-231 cells by qRT-PCR. (C) Cell viability was evaluated by CCK-8 assay in MDA-MB-468 and MDA-MB-231 cells transfected with si-PCAT6 or si-NC and/or treated with 4Gy irradiation. (D) Flow cytometry analysis was employed to determine the apoptosis rate of MDA-MB-468 and MDA-MB-231 cells transfected with si-PCAT6 or si-NC and/or treated with 4Gy irradiation. (E) Colony formation assay was applied to detect colony survival rate 14 days after MDA-MB-468 and MDA-MB-231 cells transfected with si-PCAT6 or si-NC and exposed to different doses of irradiation. *P<0.05.