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. 2020 Apr 8;13:3025–3037. doi: 10.2147/OTT.S237559

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© 2020 Shi et al.

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TPD52 was a downstream target of miR-185-5p in TNBC cells. (A) The putative binding sites between miR-185-5p and TPD52 were predicted by TargetScan. (B) Relative luciferase activity was determined in MDA-MB-468 and MDA-MB-231 cells co-transfected with TPD52-wt or TPD52-mut and NC or miR-185-5p. (C) Relative luciferase activity was detected in MDA-MB-468 and MDA-MB-231 cells co-transfected with TPD52-wt or TPD52-mut and anti-NC or anti-miR-185-5p. (D) Western blot was used to evaluate the protein level of TPD52 in MDA-MB-468 and MDA-MB-231 cells transfected with NC, miR-185-5p, anti-NC, or anti-miR-185-5p. *P<0.05.