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. 2020 Apr 8;13:3025–3037. doi: 10.2147/OTT.S237559

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© 2020 Shi et al.

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Effect of TPD52 on radiosensitivity of TNBC cells. (A) The expression of TPD52 was evaluated using qRT-PCR in TNBC tissues and matched adjacent non-tumor tissues. (B) The abundance of TPD52 was determined in tissues from TNBC patients with or without metastasis. (C) The level of TPD52 was examined in tissues from TNBC patients at different clinical stages. (D) The correlation between TPD52 mRNA level and miR-185-5p expression was analyzed in TNBC tissues. (E) The mRNA and protein levels of TPD52 were analyzed in TNBC cells (MDA-MB-468 and MDA-MB-231) and breast epithelial cells (MCF-10A) by qRT-PCR and Western blot assays, respectively. (F) The transfection efficiency of si-TPD52 was assessed using the Western blot analysis in MDA-MB-468 and MDA-MB-231 cells. (G) CCK-8 analysis was used to analyze the cell viability in MDA-MB-468 and MDA-MB-231 cells transfected with si-TPD52 or si-TPD52TPD52 and/or exposed to 4Gy irradiation. (H) Cell apoptosis was determined using the flow cytometry analysis in MDA-MB-468 and MDA-MB-231 cells transfected with si-TPD52 or si-TPD52 and/or treated with 4Gy irradiation. *P<0.05.